The aim of this study was to evaluate the clinical and radiographic outcomes of upper thoracic (UT) versus lower thoracic (LT) upper instrumented vertebrae (UIV) for adult scoliosis by meta-analysis. We conducted a literature search in three databases to retrieve related studies up to March 15, 2017. The preliminary screened studies were assessed by two reviewers according to the selection criteria. All analyses were carried out using the statistical software package R version 2.31. Odds ratios (OR) with 95% confidence intervals (CI) were used to describe the results. The I2 statistic and Q statistic test were used for heterogeneity assessment. Egger's test was performed to detect publication bias. To assess the effect of each study on the overall pooled OR or standardized mean difference (SMD), sensitive analysis was conducted. Ten trials published between 2007 and 2015 were eligible and included in our study. Meta-analysis revealed that the UT group was associated with more blood loss (SMD=0.4779, 95%CI=0.3349-0.6209, Z=6.55, P<0.0001) and longer operating time (SMD=0.5780, 95%CI=0.1971-0.958, Z=2.97, P=0.0029) than the LT group. However, there was no significant difference in Oswestry Disability Index, Scoliosis Research Society (SRS) function subscores, radiographic outcomes including sagittal vertical axis, lumbar lordosis, and thoracic kyphosis, length of hospital stay, and revision rates between the two groups. No evidence of publication bias was found between the two groups. Fusion from the lower thoracic spine (below T10) has as advantages a shorter operation time and less blood loss than upper thoracic spine (above T10) in posterior long-segment fixation for degenerative lumbar scoliosis.

Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method. The bioactivity of the released BMP-2 and VEGF was then quantified in rat mesenchymal stem cells (rMSCs). Immunoblotting analysis showed that BMP-2/PELA/VEG promoted the differentiation of rMSCs into osteoblasts via the MAPK and Wnt pathways. Osteoblast differentiation was assessed through alkaline phosphatase expression. When compared with simple BMP-2 plus VEGF group and pure PELA group, osteoblast differentiation in BMP-2/PELA/VEGF group significantly increased. An MTT assay indicated that BMP-2-loaded PELA scaffolds had no adverse effects on cell activity. BMP-2/PELA/VEG promoted the differentiation of rMSCs into osteoblast via the ERK1/2 and Wnt pathways. Our findings indicate that the sequential release of BMP-2 and VEGF from PELA microcapsule-based scaffolds is a promising approach for the treatment of bone defects.

Propofol is a frequently used intravenous anesthetic agent. Recent studies show that propofol exerts a number of non-anesthetic effects. The present study aimed to investigate the effects of propofol on lung cancer cell lines H1299 and H1792 and functional role of microRNA (miR)-486 in these effects. H1299 and/or H1792 cells were treated with or without propofol and transfected or not with miR-486 inhibitor, and then cell viability and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry. The expression of miR-486 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) with or without propofol treatment. Western blot was performed to analyze the protein expression of Forkhead box, class O (FOXO) 1 and 3, Bcl-2 interacting mediator of cell death (Bim), and pro- and activated caspases-3. Results showed that propofol significantly increased the miR-486 levels in both H1299 and H1792 cells compared to untreated cells in a dose-dependent manner (P<0.05 or P<0.01). Propofol statistically decreased cell viability but increased the percentages of apoptotic cells and protein expressions of FOXO1, FOXO3, Bim, and pro- and activated caspases-3; however, miR-486 inhibitor reversed the effects of propofol on cell viability, apoptosis, and protein expression (P<0.05 or P<0.01). In conclusion, propofol might be an ideal anesthetic for lung cancer surgery by effectively inhibiting lung cancer cell viability and inducing cell apoptosis. Modulation of miR-486 might contribute to the anti-tumor activity of propofol.

In deep excavations under conditions of high ground stress, even hard rock undergoes creep and other time-dependent effects that may result in instability. This study involves an analysis of the typical damage to the surrounding rock in the deep tunnels of the Jinchuan nickel mine in China. In order to understand the creep mechanism, triaxial creep laboratory tests were conducted under high stress. The creep characteristic curves and the Nishihara model parameters of typical rock were obtained from these tests. The numerical simulations, with which the creep model is developed, were performed using Flac3D. The creep model parameters were back-analysed on the basis of field monitoring data. Finally, appropriate support schemes for deep tunnels are suggested. The creep deformation of rock increases with time and axial load. At the same load level, the creep deformation of unloading is greater than that of loading. The simulated deformations of the surrounding rock are coincident with those from in situ monitoring, indicating the applicability of the presented rock creep model in situations of high ground stress. The results constitute a reference for support design in deep mining tunnels. rock creep, high ground stress, deformation, modelling, numerical simulation.

The traditional SWOT tool, which lists the S.W.O.T., factors and groups them together to form some strategies, is convenient for analyzing but not effective enough for strategic formulation. This paper proposes a hybrid "three-stage" qualitative and quantitative SWOT model, trying to narrow the gap between strategic analysis and strategic formulation by first confirming the SWOT factors, second narrowing the selection area and last making optimal strategies. First, the SWOT-AHP model is used to analyze and evaluate the external and internal environment factors. Then we narrow the analysis field down to two levels by using strategic quadrilateral model, where the quadrant of the gravity centre is used to represent the market position. Finally correlation rules, including matrix calculations, maximum and sub-maximum sub-array techniques, are introduced to formulate the effective strategies, which focus on the most influential factors. A case study of EMS is conducted with the objective of validating the effectiveness in strategic planning and management.

Prolactin (PRL), a polypeptide hormone synthesized and secreted by the animal's anterior pituitary gland, plays an important role in the regulation of mammalian lactation and avian reproduction. Considering the significant association between single nucleotide polymorphisms (SNPs) in the 5'-flanking region of PRL and reproduction traits in the chicken, the objective of this study was to screen for SNP in the 5'-proximal region of PRL in geese and to evaluate the association between SNP and reproduction traits in geese. Chinese Wan-xi White geese (n = 200) and European Rhine geese (n = 80) were used for phenotyping. SNP was screened by comparing sequences of PCR products, and the single-stranded conformational polymorphism (SSCP) protocol was adopted for genotyping. Three SNPs (A-401G, G-268A and T-266A) in the 5'-proximal region of goose PRL were identified in both breeds. Statistical analysis suggested that the genotype AA characterized by A-401, G-268 and T-266 had a positive genetic effect on egg production. Therefore, these polymorphisms have the potential to be utilized in molecular breeding for egg production in geese.